Approximately 5 105 cells/well were inoculated in 6-well plates, collected by centrifugation, suspended in phosphate buffered saline (PBS), and stained with JC-1 for 20 min (37C)
Once taken up by copper transporter 1 (CTR1), Cu2+ is transported to different organelles in the cytoplasm and is metabolized by a copper chaperone for cytochrome c oxidase [CCO
GPx6, interestingly, is selenoprotein in humans but cysteine-based in some animals, showing evolutionary tweaks
Signal Transduct Target Ther 9(1):17 Tariot PN, Cummings JL, Katz IR, Mintzer J, Perdomo CA, Schwam EM, Whalen E (2001) A randomized, double-blind, placebo-controlled study of the efficacy and safety of donepezil in patients with Alzheimers disease in the nursing home setting